C/EBP homologous protein (CHOP) up‐regulates IL‐6 transcription by trapping negative regulating NF‐IL6 isoform

FEBS Letters

Onozaki, Kikuo¹  Hayashi, Hidetoshi¹  Hattori, Takayuki¹  Ohoka, Nobumichi¹ 

[1] Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho, Nagoya 467-8603, Japan

关键词: C/EBP homologous protein;    Nuclear factor for IL-6 expression;    Liver-enriched inhibitory protein;    Interleukin-6;    Transcription;    Dimer;    AP-1;    activator protein 1;    C/EBP;    CCAAT/enhancer binding protein;    ChIP;    chromatin immunoprecipitation;    CHOP;    C/EBP homologous protein;    CRP;    C-reactive protein;    G-CSF;    granulocyte colony stimulating factor;    GST;    glutathione S-transferase;    IL;    interleukin;    LAP;    liver-enriched activator protein;    LIP;    liver-enriched inhibitory protein;    NF-IL6;    nuclear factor for IL-6 expression;    NF-κB;    nuclear factor κB;    PCR;    polymerase chain reaction;    SDS–PAGE;    sodium dodecyl sulfate–polyacrylamide gel electrophoresis;    TNF;    tumor necrosis factor;   

DOI  :  10.1016/S0014-5793(03)00283-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interleukin-6 (IL-6) production is up-regulated by several stimuli through the activation of transcription factors. We have previously demonstrated that CCAAT/enhancer binding protein homologous protein (CHOP) positively regulates IL-6 production at the transcriptional level in the human melanoma cell line A375. In this study, we provide evidence that CHOP up-regulates the IL-6 transcription without binding to the IL-6 promoter. CHOP dimerized more preferentially with an inhibitory isoform of nuclear factor for IL-6 expression (LIP (liver-enriched inhibitory protein)) than with a positively acting isoform (LAP, liver-enriched activator protein). These results indicate that CHOP plays an important role in IL-6 production without binding to its promoter, probably by trapping protein(s) such as LIP, which would otherwise inhibit IL-6 transcription.

【 授权许可】

Unknown