Unexpected catalytic site variation in phosphoprotein phosphatase homologues of cofactor‐dependent phosphoglycerate mutase

FEBS Letters

Rigden, Daniel J.¹ 

[1] Embrapa Genetic Resources and Biotechnology, Cenargen/Embrapa, Parque Estação Biológica, Final W3 Norte, 70770-900 Brası́lia, Brazil

关键词: Cofactor-dependent phosphoglycerate mutase;    Fructose-2;    6-bisphosphatase;    Evolutionary relationship;    Sequence analysis;   

DOI  :  10.1016/S0014-5793(03)00014-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cofactor-dependent phosphoglycerate mutase (dPGM) superfamily contains, besides mutases, a variety of phosphatases, both broadly and narrowly substrate-specific. Distant dPGM homologues, conspicuously abundant in microbial genomes, represent a challenge for functional annotation based on sequence comparison alone. Here we carry out sequence analysis and molecular modelling of two families of bacterial dPGM homologues, one the SixA phosphoprotein phosphatases, the other containing various proteins of no known molecular function. The models show how SixA proteins have adapted to phosphoprotein substrate and suggest that the second family may also encode phosphoprotein phosphatases. Unexpected variation in catalytic and substrate-binding residues is observed in the models.

【 授权许可】

Unknown