Spinach leaf 6‐phosphofructo‐2‐kinase

FEBS Letters

Huber, Steven C.¹  Walker, Griffin H.¹ 

[1] United States Department of Agriculture, Agriculture Research Service, and Departments of Botany and Crop Science, North Carolina State University, Raleigh, NC 27650, USA

关键词: Regulation;    Pi ATP;    Fructose-2;    6-bisphosphatase;    6-phosphofructo-2-kinase;    (Spinacia oleracea L.);    F26BP;    fructose 2;    6-bisphosphate;    F6P;    2K;    6-phosphofructo-2-kinase;    F26BPase;    fructose-2;    6-bisphosphatase;    F6P;    fructose 6-phosphate;    G6P;    glucose 6-phosphate;    PMSF;    phenylmethylsulfonyl fluoride;    BAD;    benzamidine;   

DOI  :  10.1016/0014-5793(87)81525-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel form of 6-phosphofructo-2-kinase was partially purified from spinach (Spinacia oleracea L.) leaves. As isolated, the new enzyme form possessed little or no enzymatic activity. However, pretreatment with 2 mM Mg-ATP dramatically increased the activities of 6-phosphofructo-2-kinase and fructose-2,6-bisphosphatase. Activation only occurred in the presence of magnesium plus ATP; either alone was ineffective. The ATP activation was reversed by treatment with alkaline phosphatase and could be completely restored by subsequent incubation with Mg-ATP. Thus, protein phosphorylation appears to be the mechanism involved. This is the first evidence that higher plants contain an interconvertible form of the enzyme.

【 授权许可】

Unknown