Identification and characterization of Mlr1,2: two mouse homologues of Mblk‐1, a transcription factor from the honeybee brain

FEBS Letters

Takeuchi, Hideaki¹  Kunieda, Takekazu¹  Kubo, Takeo²  Park, Jung-Min² 

[1] Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19, Toranomon, Minato-ku, Tokyo 105-0001, Japan;Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan

关键词: Honeybee;    Brain;    Transcription factor;    Spermatocytes;    Spermatogenesis;    E93;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(02)03858-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously identified the Mblk-1 gene in the honeybee brain, which encodes a transcription factor containing two DNA binding motifs, termed RHF1 and 2 (Takeuchi et al. (2001) Insect Mol. Biol. 121, 134–140). Here, we identified two mouse Mblk1 homologues, Mlr1 and Mlr2. Both encode proteins containing a single DNA-binding motif highly conserved with RHF2 and activate transcription mediated by a DNA element recognized by honeybee Mblk-1. Mlr1 was expressed predominantly in the spermatocytes of the testis, while Mlr2 was expressed in various tissues other than testis. Mlr1 transcripts were lost in the testis of W/W^v mutant mice, suggesting a role in spermatogenesis.

【 授权许可】

Unknown