Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray 1

FEBS Letters

Fujiyuki, Tomoko²  Kato, Azusa¹  Kamikouchi, Azusa²  Shirai, Kenichi²  Takeuchi, Hideaki³  Matsuo, Yuko²  Tsujimoto, Atsumi¹  Fujinawa, Yumi¹  Kubo, Takeo² 

[1] DNA Chip Research Inc., 1-1-34 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan;Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan;Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19, Toranomon, Minato-ku, Tokyo 105-0001, Japan

关键词: Honeybee;    Mushroom body;    cDNA microarray;    Differential display;    Inositol 1;    4;    5-trisphosphate 5-phosphatase;    Brain;   

DOI  :  10.1016/S0014-5793(02)02319-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP₃) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP₃-mediated Ca²⁺ signaling is enhanced in these neurons.

【 授权许可】

Unknown