Spermatocyte‐specific expression of the gene for mouse testis‐specific transcription elongation factor S‐II

FEBS Letters

Natori, Shunji¹  Takeuchi, Hideaki¹  Xu, Qihong¹  Ito, Takahiro¹  Kubo, Takeo¹ 

[1] Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Transcription elongation factor;    S-11;    SII-TI;    Spermatocytes;    Spermatogenesis;   

DOI  :  10.1016/0014-5793(96)00340-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previously, we characterized a rat cDNA for testisspecific transcription elongation factor S-II (SII-T1) (Q. Xu et al., J. Biol. Chem. 269, 3100–3103 (1994)). Here, we isolated a 335-bp fragment of the cDNA for mouse SII-T1, and used it to examine the expression of the SII-T1 gene in the testis by in situ hybridization. The results indicated that the SII-T1 gene is expressed exclusively in spermatocytes, showing no appreciable expression in spermatogonia, spermatids, or Leydig cells. RTPCR experiments using testis RNA from WI W^v mutant mice also suggested that SII-T1 is a specific transcription elongation factor essential for spermatogenesis.

【 授权许可】

Unknown