Mice overexpression of human augmenter of liver regeneration (hALR) in male germ cells shows abnormal spermatogenesis and reduced fertility

Endocrine Journal

Xiao-Hui Wang¹  Chang-Yan Li¹  Zhi-Dong Wang¹  Miao Yu¹  Xiao-Ming Yang¹  Wang-Xiang Xu¹  Yu-Long Fu¹  Wei Li¹  Yan Cao¹  Chang-Hui Ge¹  Yi-Qun Zhan¹ 

[1] Beijing Institute of Radiation Medicine, Beijing, China

关键词: Augmenter of liver regeneration;    Spermatocytes;    Apoptosis;    Male fertility;   

DOI  :  10.1507/endocrj.EJ12-0160

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(40)Cited-By(2)Human augmenter of liver regeneration (hALR) is a sulfhydryl oxidase that is highly expressed in spermatogonia and early spermatocytes.To investigate the physiological effects of hALR in spermatogenesis, we generated a hALR transgenic mouse model driven by the human TSPY (testis-specific protein, Y-encoded) promoter that allows the transgene to be specifically activated in the testes.hALR content was found to be increased in both germ cells.The histological and TUNEL analysis of transgenic testes revealed a number of spermatogenetic defects including primary spermatocyte overpopulation followed by depletion through apoptosis, degenerating and detached nucleated germ cells, haploid cell loss and intraepithelial vacuoles of varying sizes.In line with these features, adult transgenic male mice also displayed a reduction in fertility.Our data suggest that regulated spatial and temporal expression of hALR is required for normal testicular development and spermatogenesis, and overexpression of hALR results in influencing the sperm morphology and quantity and the eventual reduction in male fertility.Present findings in the mouse may be of interest to human male fertility.

【 授权许可】

Unknown