Tyrosine kinase‐type receptor ErbB4 in chondrocytes: interaction with connective tissue growth factor and distribution in cartilage

FEBS Letters

Yosimichi, Gen²  Kuboki, Takuo⁴  Kubota, Satoshi²  Yatani, Hirofumi⁴  Kanyama, Manabu⁴  Nishida, Takashi²  Takigawa, Masaharu²  Nakanishi, Tohru²  Nawachi, Kumiko²  Inoue, Miho³  Yamaai, Tomoichiro¹ 

[1] Department of Oral Functional Anatomy, Okayama University Graduate School Medicine and Dentistry, Okayama 700-8525, Japan;Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8525, Japan;Biodental Research Center, Okayama University Dental School, Okayama 700-8525, Japan;Department of Oral and Maxillofacial Rehabilitation, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8525, Japan

关键词: Connective tissue growth factor;    Receptor;    Chondrocyte;    Cartilage;    ErbB4;   

DOI  :  10.1016/S0014-5793(02)03263-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to identify receptor molecules that participate in the growth and differentiation of chondrocytes, we cloned a number of cDNA fragments from HCS-2/8 chondrocytic cells, by using tyrosine kinase-specific primers for amplification. The mRNA expression of one such receptor, ErbB4, was increased by connective tissue growth factor/hypertrophic chondrocyte-specific gene product (CTGF/Hcs24), which promotes all stages of the endochondral ossification in vitro. ErbB4 expression was observed through all stages of chondrocytic differentiation in vitro, corresponding to the wide distribution of CTGF/Hcs24 target cells. Furthermore, positive signals for erbB4 mRNA were detectable throughout most populations of chondrocytes, in growth and articular cartilage in vivo. These results demonstrate for the first time that ErbB4 is expressed in chondrocytes and may play some roles in chondrocytic growth and differentiation along with CTGF/Hcs24.

【 授权许可】

Unknown