Novel intracellular effects of human connective tissue growth factor expressed in Cos‐7 cells

FEBS Letters

Kubota, Satoshi¹  Takigawa, Masaharu¹  Shimo, Tsuyoshi²  Nakanishi, Tohru¹  Hattori, Takako¹ 

[1] Department of Biochemistry and Molecular Dentistry, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan;Department of Oral and Maxillofacial Surgery II, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan

关键词: Connective tissue growth factor;    Chondrocyte;    Cell cycle arrest;   

DOI  :  10.1016/S0014-5793(00)01573-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. In Cos-7 cells, expression of human CTGF induced an altered cell morphology. It was associated with an increased cellular DNA content and loose attachment, indicating the cells were in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly. These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery.

【 授权许可】

Unknown