Involvement of cis‐acting repressive element(s) in the 3′‐untranslated region of human connective tissue growth factor gene

FEBS Letters

Kubota, Satoshi¹  Takigawa, Masaharu¹  Nakanishi, Tohru¹  Hattori, Takako¹ 

[1] Department of Biochemistry and Molecular Dentistry, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan

关键词: Connective tissue growth factor;    Chondrocyte;    Cartilage;    3′-Untranslated region;    Gene expression;    CTGF;    connective tissue growth factor;    3′-UTR;    3′-untranslated region;    PDGF;    platelet-derived growth factor;    BMP-2;    bone morphogenic protein 2;    TGF-β;    transforming growth factor β;    MCS;    multiple cloning site;    RT-PCR;    reverse transcriptase-mediated polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(99)00480-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To analyze the regulatory mechanism of connective tissue growth factor expression, the 3′-untranslated region (3′-UTR) of CTGF cDNA was amplified from HeLa cell RNA. Direct nucleotide sequencing revealed a single major population in the amplicon, which was nearly identical to other sequences. Subsequently, the effect of the 3′-UTR on gene expression was evaluated. When it was fused downstream of a firefly luciferase gene, the 3′-UTR strongly repressed luciferase gene expression. Interestingly, the repressive effect of the antisense 3′-UTR appeared to be more prominent than that of the sense one. Together with the fact that several consensus sequences for regulatory elements are found in it, these results suggest the involvement of multiple sets of regulatory elements in the CTGF 3′-UTR.

【 授权许可】

Unknown