Effects of copper on the expression of metal transporters in human intestinal Caco‐2 cells

FEBS Letters

Sharp, Paul¹  Tennant, Jason¹  Stansfield, Matthew¹  Yamaji, Sachie²  Srai, Surjit Kaila² 

[1] Centre for Nutrition and Food Safety, School of Biomedical and Life Sciences, University of Surrey, Guildford GU2 7XH, UK;Department of Biochemistry and Molecular Biology, Royal Free and University College Medical School, London NW3 2PF, UK

关键词: DMT1;    IREG1;    Ctr1;    Copper;    Caco-2 cell;    DMT1;    divalent metal transporter;    IREG1;    iron-regulated mRNA;    IRE;    iron responsive element;    UTR;    untranslated region;    RT-PCR;    reverse transcription-polymerase chain reaction;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid;   

DOI  :  10.1016/S0014-5793(02)03253-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Copper is an essential dietary trace metal, however the mechanisms involved in intestinal copper uptake are unclear. Two putative copper transporters are expressed in Caco-2 cells, the divalent metal transporter (DMT1) and copper transporter (Ctr1). Our data demonstrate that copper could compete with iron for uptake via DMT1 and that DMT1 protein and mRNA expression were decreased following exposure (24 h) to high copper. Expression of Ctr1, which acts as a copper transporter in transfected cell lines, was unaffected by copper treatment. Interestingly, exposure to copper increased iron efflux from Caco-2 cells and up regulated IREG1 (iron-regulated mRNA) expression.

【 授权许可】

Unknown