Zinc regulates the function and expression of the iron transporters DMT1 and IREG1 in human intestinal Caco‐2 cells

FEBS Letters

Williams, Mark¹  Sharp, Paul²  Tennant, Jason²  Singh Srai, Surjit Kaila³  Yamaji, Sachie³  Tandy, Sarah¹ 

[1] School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK;Centre for Nutrition and Food Safety, School of Biomedical and Life Sciences, University of Surrey, Guildford GU2 7XH, UK;Department of Biochemistry and Molecular Biology, Royal Free and University College Medical School, London NW3 2PF, UK

关键词: Divalent metal transporter;    IREG1;    Iron transport;    Caco-2 cell;    Zinc;    DMT1;    divalent metal transporter;    IREG1;    iron-regulated mRNA;    RT-PCR;    reverse transcription polymerase chain reaction;    IRE;    iron responsive element;    IRP;    iron regulatory protein;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid;   

DOI  :  10.1016/S0014-5793(01)02953-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Trace metals influence the absorption of each other from the diet and it has been suggested that the divalent metal transporter (DMT1) represents a common uptake pathway for these important micronutrients. However, compelling evidence from our laboratory suggests that DMT1 is predominantly an iron transporter, with lower affinity for other metals. Several studies have shown that increasing dietary iron downregulates DMT1. Interestingly, our current data indicate that zinc upregulates DMT1 protein and mRNA expression and also pH-dependent iron uptake. Transepithelial flux of iron was also increased and was associated with a rise in IREG1 mRNA expression.

【 授权许可】

Unknown