Genome‐wide studies on the nuclear PDR3‐controlled response to mitochondrial dysfunction in yeast

FEBS Letters

Carvajal, Elvira¹  Moye-Rowley, Scott³  Jacq, Claude²  Devaux, Frédéric² 

[1] Departamendo de Biologia Celular e Genetica, Universidade do Estado do Rio de Janeiro, Rua Sao Francisco Xavier 524, CEP 20550-013, Rio de Janeiro, Brazil;Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France;Department of Physiology and Biophysics, Bowen Science Building, University of Iowa, Iowa City, IA 52242, USA

关键词: Yeast;    Microarray;    Drug resistance;    Mitochondrion;    PDR;    pleiotropic drug resistance;   

DOI  :  10.1016/S0014-5793(02)02387-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gain-of-function mutations in the transcription factors Pdr1p and Pdr3p lead to the up-regulation of genes controlling plasma membrane properties. Pdr3p is involved in a retrograde response in which mitochondrial dysfunctions activate PDR5, a gene encoding an ABC membrane transporter. We carried out genome-wide analyses of the PDR3-controlled genes activated by the deletion of the mitochondrial DNA. We present evidence showing that PDR1 does not interfere with this PDR3 response. We also showed that the mitochondrially activated PDR3 response is highly sensitive to both yeast strain variations and carbon sources. These observations explain the apparent discrepancies in published studies and better describe the connections between the mitochondrial state and plasma membrane properties.

【 授权许可】

Unknown