Identification of the mitochondrial carnitine carrier in Saccharomyces cerevisiae

FEBS Letters

Iacobazzi, Vito²  Runswick, Michael J.¹  Lasorsa, Francesco M.²  Walker, John E.¹  Palmieri, Luigi²  Palmieri, Ferdinando² 

[1] The M.R.C. Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2DH, UK;Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via Orabona 4, 70125 Bari, Italy

关键词: Yeast;    Mitochondrion;    Transport;    CRC1 gene;    Carnitine carrier;    YOR100c;   

DOI  :  10.1016/S0014-5793(99)01555-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mitochondrial carrier protein for carnitine has been identified in Saccharomyces cerevisiae. It is encoded by the gene CRC1 and is a member of the family of mitochondrial transport proteins. The protein has been over-expressed with a C-terminal His-tag in S. cerevisiae and isolated from mitochondria by nickel affinity chromatography. The purified protein has been reconstituted into proteoliposomes and its transport characteristics established. It transports carnitine, acetylcarnitine, propionylcarnitine and to a much lower extent medium- and long-chain acylcarnitines.

【 授权许可】

Unknown