The human homologue of the yeast mitochondrial AAA metalloprotease Yme1p complements a yeast yme1 disruptant

FEBS Letters

de Jong, Lisbeth²  Grivell, Leslie A.²  Shah, Zahid H.¹  Jacobs, Howard T.¹  Hakkaart, Gerrit A.J.¹  van der Spek, Hans²  Arku, Benedict¹ 

[1] Institute of Medical Technology and Tampere University Hospital, P.O. Box 607, 33101 Tampere, Finland;Swammerdam Institute for Lifescience, Section for Molecular Biology, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands

关键词: Metalloprotease;    AAA protein;    ATPase;    Yeast;    Mitochondrion;    YME1;   

DOI  :  10.1016/S0014-5793(00)01859-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L gene on chromosome 10p. Transfection of both HEK-293EBNA and yeast cells with a green fluorescent protein-tagged YME1L cDNA confirmed mitochondrial targeting. When expressed in a yme1 disruptant yeast strain, YME1L restored growth on glycerol at 37°C. We propose that YME1L plays a phylogenetically conserved role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies.

【 授权许可】

Unknown