FEBS Letters | |
In vivo glucose activation of the yeast plasma membrane ATPase | |
Serrano, Ramón1  | |
[1] Instituto de Enzimología y Patología Molecular del CSIC, Facultad de Medicina de la Universidad Autónoma, Calle Arzobispo Morcillo no. 4, Madrid-34, Spain | |
关键词: ATPase; Plasma membrane; Yeast; Proton transport; Regulation; Interconvertible enzyme; | |
DOI : 10.1016/0014-5793(83)80237-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The addition of glucose to yeast cells activates proton efflux mediated by the plasma membrane ATPase. Accordingly, the ATPase activity of purified plasma membranes is increased up to 10-fold. The activated ATPase has a more alkaline pH optimum, better affinity for ATP and greater sensitivity to vanadate than the non-activated enzyme. All these changes are reversed by washing the cells free of glucose. This suggests two states of the ATPase which are interconverted by a covalent modification. As glucose does not affect the phosphorylation of plasma membrane polypeptides, other type of covalent modification may be involved.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020284276ZK.pdf | 354KB | download |