In vivo glucose activation of the yeast plasma membrane ATPase

FEBS Letters

Serrano, Ramón¹ 

[1] Instituto de Enzimología y Patología Molecular del CSIC, Facultad de Medicina de la Universidad Autónoma, Calle Arzobispo Morcillo no. 4, Madrid-34, Spain

关键词: ATPase;    Plasma membrane;    Yeast;    Proton transport;    Regulation;    Interconvertible enzyme;   

DOI  :  10.1016/0014-5793(83)80237-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The addition of glucose to yeast cells activates proton efflux mediated by the plasma membrane ATPase. Accordingly, the ATPase activity of purified plasma membranes is increased up to 10-fold. The activated ATPase has a more alkaline pH optimum, better affinity for ATP and greater sensitivity to vanadate than the non-activated enzyme. All these changes are reversed by washing the cells free of glucose. This suggests two states of the ATPase which are interconverted by a covalent modification. As glucose does not affect the phosphorylation of plasma membrane polypeptides, other type of covalent modification may be involved.

【 授权许可】

Unknown