Cyclic AMP controls the plasma membrane H+‐ATPase activity from Saccharomyces cerevisiae

FEBS Letters

Goffeau, André²  Ulaszewski, Stanislaw²  Hilger, François¹ 

[1] Service de Microbiologie, Faculté des Sciences Agronomiques de l'Etat, B-5800 Gembloux, Belgium;Laboratoire d'Enzymologie, Université Catholique de Louvain, B-1348 Louvain-la-Neuve Belgium

关键词: ATPase;    H+-;    cyclic AMP;    Plasma membrane;    Cell cycle;    ATPase;    mitochondrial;    (Saccharomyces cerevisiae);   

DOI  :  10.1016/0014-5793(89)80206-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The thermosensitive G₁-arrested cdc35-10 mutant from Saccharomyces cerevisiae, defective in adenylate cyclase activity, was shifted to restrictive temperature. After 1 h incubation at this temperature, the plasma membrane H⁺-ATPase activity of cdc35-10 was reduced to 50%, whereas that in mitochondria doubled. Similar data were obtained with cdc25, another thermosensitive G₁-arrested mutant modified in the cAMP pathway. In contrast, the ATPase activities of the G₁-arrested mutant cdc19, defective in pyruvate kinase, were not affected after 2 h incubation at restrictive temperature. In the double mutants cdc35-10 cas1 and cdc25 cas1, addition of extracellular cAMP prevented the modifications of ATPase activities observed in the single mutants cdc35-10 and cdc25. These data indicate that cAMP acts as a positive effector on the H⁺-ATPase activity of plasma membranes and as a negative effector on that of mitochondria.

【 授权许可】

Unknown