ATP stimulates the uptake of S‐dinitrophenylglutathione by rat liver plasma membrane vesicles

FEBS Letters

Sogame, Yoshihisa²  Nicotera, Pierluigi¹  Hayashi, Koichiro²  Orrenius, Sten¹  Kobayashi, Kazuo² 

[1] Department of Toxicology, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden;Institute of Scientific and Industrial Research, Osaka University, Ibaraki 567, Japan

关键词: Glutathione;    Hepatocyte;    Plasma membrane;    ATPase;   

DOI  :  10.1016/0014-5793(88)80339-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of inverted plasma membrane vesicles from rat liver with micromolar concentrations of S-dinitrophenylglutathione (DNP-SG) in the presence of ATP resulted in the uptake of DNP-SG into the vesicles. ATP-dependent DNP-SG accumulation was half-maximal with 9 μM DNP-SG, while the K _m for ATP was 320 μM. Glutathione disulfide (GSSG), but not reduced glutathione, inhibited the ATP-dependent accumulation of DNP-SG by the vesicles, suggesting that the same, ATP-dependent transport system is responsible for the extrusion of glutathione conjugates and GSSG from liver cells.

【 授权许可】

Unknown