Inhibition of hepatocyte plasma membrane Ca2+‐ATPase activity by menadione metabolism and its restoration by thiols

FEBS Letters

Bellomo, Giorgio¹  Nicotera, Pierluigi¹  Mirabelli, Francesca¹  Moore, Margo¹  Orrenius, Sten¹ 

[1] Department of Toxicology, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden

关键词: Hepatocyte;    Plasma membrane;    Ca2+-ATPase;    Menadione;    Glutathione;    Thiol;    menadione;    2-methyl-l;    4-naphthoquinone;    GSH;    glutathione (reduced form);    DTT;    dithiothreitol;    Hepes;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    EGTA;    ethylene bis (β-aminoethyl ether)-N;    N;    N ';    N'-tetraacetic acid;    NEM;    N-ethylmaleimide;    DTNB;    2;    2'-dinitro-5;    5'-dithiodibenzoic acid;   

DOI  :  10.1016/0014-5793(85)81131-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of isolated rat hepatocytes with cytotoxic concentrations of menadione resulted in inhibition of plasma membrane Ca²⁺-ATPase activity. This could be restored by subsequent treatment with either dithiothreitol or reduced glutathione, suggesting that the inhibition by menadione was due to oxidation of sulfhydryl groups critical for Ca²⁺-ATPase activity.

【 授权许可】

Unknown