Electrogeneicity of the lysosomal proton pump

FEBS Letters

Dell'Antone, Paolo¹ 

[1] CNR Unit for the Study of Physiology of Mitochondria, c/o Institute of General Pathology, University of Padova, Italy

关键词: Lysosome;    Proton transport;    ATPase;    Acridine orange;    Electrogenic proton pump;    (Rat liver);    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;   

DOI  :  10.1016/0014-5793(84)80198-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using acridine orange as a pH gradient probe, the effects of valinomycin and FCCP on the pH gradient across lysosomal membranes in an ATP-free medium as well as on the rate of the inward ATP-driven proton translocation were investigated. Both lysosome-enriched and highly purified lysosomal preparations from rat liver were used with identical results. Ionophore effects were found to be different depending upon whether passive ion fluxes or ATP-driven H⁺ translocation were involved and supported the existence of a membrane potential in the latter case. Anions stimulated the rate of ATP-driven proton translocation and stimulation increased with increasing anion lipophilicity. These results strongly support the electrogenic nature of the lysosomal proton pump.

【 授权许可】

Unknown