Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP‐1 family through heterologous expression in yeast

FEBS Letters

Vandenberg, Carol A.²  Liang, Hong¹  Radeke, Carolyn M.²  Coulter, Kathryn L.²  Gaber, Richard F.¹  Périer, François² 

[1] Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA;Department of Biological Sciences and Neuroscience Research Institute University of California Santa Barbara, CA 93106, USA

关键词: ATPase;    Membrane fusion;    Yeast;    Suppressor;    Potassium transport;    TRK;   

DOI  :  10.1016/0014-5793(94)00879-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (uppressor of ⁺ transport growth efect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N-ethylmaleimide-sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP-1). The SKD1 protein constitutes a novel member of this family with 49–58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.

【 授权许可】

Unknown