Chickens’ Cry2: molecular analysis of an avian cryptochrome in retinal and pineal photoreceptors

FEBS Letters

Cassone, Vincent M¹  Bailey, Michael J¹  Chong, Nelson W²  Xiong, Jin³ 

[1]Biological Clocks Program, Texas A&M University, College Station, TX, USA

[2]Centre for Chronobiology, School of Biomedical and Life Sciences, University of Surrey, Guildford, UK

[3]Department of Biology, Texas A&M University, College Station, TX, USA

关键词: Circadian rhythm;    Cryptochrome;    Pineal gland;    Retina;    Chicken;    Clock gene;    cry;    cryptochrome;    SCN;    suprachiasmatic nucleus;    LD;    light:dark;    AANAT;    arylalkylamine N-acetyltransferase;    per;    period;    clk;    clock;    bmal1;    brain muscle ARNT-like protein 1;    ZT;    Zeitgeber time;    TeO;    optic tectum;    GCL;    ganglion cell layer;    RBP;    retinol-binding protein;   

DOI  :  10.1016/S0014-5793(02)02276-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified and characterized an ortholog of the putative mammalian clock gene cryptochrome 2 (Cry2) in the chicken, Gallus domesticus. Northern blot analysis of gCry2 mRNA indicates widespread distribution in central nervous and peripheral tissues, with very high expression in pineal and retina. In situ hybridization of chick brain and retina reveals expression in photoreceptors and in visual and circadian system structures. Expression is rhythmic; mRNA levels predominate in late subjective night. The present data suggests that gCry2 is a candidate avian clock gene and/or photopigment and set the stage for functional studies of gCry2.

【 授权许可】

Unknown