Monochromatic green light stimulation during incubation shortened the hatching time via pineal function in White Leghorn eggs

Journal of Animal Science and Biotechnology

Zhengdong Yuan¹  Xiaolin Liu²  Adamu Mani Isa³  Aixin Ni³  Yanyan Sun³  Jing Fan³  Pingzhuang Ge³  Yuanmei Wang³  Yunhe Zong³  Lei Shi³  Jilan Chen³  Linlin Jiang³  Shixiong Bian³  Hui Ma³  Yunlei Li³  Panlin Wang⁴ 

[1]Beijing DQY Agricultural Science and Technology Co., Ltd., 100094, Beijing, China

[2]College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, China

[3]Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, 100193, Beijing, China

[4]Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, 100193, Beijing, China

[5]College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, China

关键词: Circadian rhythm;    Hatching time;    Insulin-like growth factor 1;    Melatonin;    Monochromatic green light;    Pineal gland;   

DOI  :  10.1186/s40104-020-00539-x

来源: Springer

【 摘 要 】

BackgroundEffect of monochromatic green light illumination on embryo development has been reported in chickens. The avian pineal gland is an important photo-endocrine organ formed by a mediodorsal protrusion during embryonic development. However, the involvement of pineal gland in the light transduction process remains to be elucidated. In the present study, we investigated the influence of monochromatic green light on hatching time and explored the possible mechanism via pineal function.ResultsA total of 600 eggs of White Leghorn (Shaver strain) were incubated under photoperiods of either 12 h of light and 12 h of darkness using monochromatic green light (12L:12D group) or 24 h of darkness (0L:24D group) for 18 d. Compared to 0L:24D group, the green light stimulation shortened the hatching time without extending the hatch window or impairing hatchability. The liver of embryos incubated in the 12L:12D light condition was heavier than those of the 0L:24D group on d 21 post incubation which may be linked to the observed increase in the serum concentration of insulin-like growth factor 1 (IGF-1); primary secretion of the liver. Histological structure analysis of pineal gland demonstrated that the light stimulation increased follicle area, wall thickness and lumen area on d 10 and d 12 post incubation. Rhythmic function analysis demonstrated that three clock related genes (brain and muscle ARNT-like-1, BMAL1; circadian locomotor output cycles kaput, CLOCK; and cryptochrome-1, CRY1) and a melatonin rate-limiting enzyme related gene (arylalkylamine N-acetyltransferase, AANAT) were rhythmically expressed in the pineal gland of the 12L:12D group, but not in the 0L:24D group. Simultaneously, the light stimulation also increased the concentration of melatonin (MT), which was linked to hepatocyte proliferation and IGF-1 secretion in previous studies.ConclusionsThe 12L:12D monochromatic green light stimulation during incubation shortened hatching time without impairing hatching performance. Pineal gland’s early histological development and maturation of its rhythmic function were accelerated by the light stimulation. It may be the key organ in the photo-endocrine axis that regulates embryo development, and the potential mechanism could be through enhanced secretion of MT in the 12L:12D group which promotes the secretion of IGF-1.

【 授权许可】

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