Adenosine stimulates neuromedin U mRNA expression in the rat pars tuberalis

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:496

Article

Aizawa, Sayaka¹  Gu, Tingting¹  Kaminoda, Arisa¹  Fujioka, Ryuya¹  Ojima, Fumiya²  Sakata, Ichiro³  Sakai, Takafumi³  Ogoshi, Maho¹  Takahashi, Sumio¹  Takeuchi, Sakae¹ 

[1] Okayama Univ, Grad Sch Nat Sci & Technol, Dept Biol, Kita Ku, 3-1-1 Tsushimanaka, Okayama 7008530, Japan

[2] Kawasaki Med Sch, Dept Nat Sci & Biol, 577 Matsushima, Kurashiki, Okayama 7010192, Japan

[3] Saitama Univ, Grad Sch Sci & Engn, Div Life Sci, Area Regulatory Biol,Sakura Ku, 255 Shimo Ohkubo, Saitama 3388570, Japan

关键词: Neuromedin U;    Pars tuberalis;    Adenosine receptor;    cAMP response element;    Circadian rhythm;    Melatonin;   

DOI  :  10.1016/j.mce.2019.110518

来源: Elsevier

【 摘 要 】

Neuromedin U (NMU) shows circadian expression in the rat pars tuberalis (PT), and is known to be suppressed by melatonin. Here we examined the involvement of adenosine in the regulation of Nmu expression. We found that the rat PT expressed adenosine receptor A2b and that an adenosine receptor agonist, NECA, stimulated Nmu expression in brain slice cultures. In vitro promoter assays revealed that NECA stimulated Nmu promoter activity via a cAMP response element (CRE) in the presence of adenosine receptor A2b. NECA also increased the levels of phosphorylated CRE-binding protein. These findings suggest that adenosine stimulates Nmu expression by activating the cAMP signaling pathway through adenosine receptor A2b in the rat PT. This is the first report to demonstrate that Nmu expression in the PT is regulated by adenosine, which acts as an intravital central metabolic signal, in addition to melatonin, which acts as an external photoperiodic environmental signal.

【 授权许可】

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