FEBS Letters | |
Identification of functional type 1 ryanodine receptors in mouse dendritic cells | |
Klyachko, Vitaly A.3  Ahern, Gerard P.1  O'Connell, Peta J.2  | |
[1] Department of Pharmacology, Georgetown University, Washington DC, 20007, USA;The John P. Robarts Research Institute, London, ON, Canada N6A 5K8;Department of Physiology and Biophysics PhD Program, University of Wisconsin-Madison, Madison, WI 53706, USA | |
关键词: Dendritic cell; Calcium; Ryanodine receptor; FK506; Caffeine; | |
DOI : 10.1016/S0014-5793(01)03321-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Ca2+ signaling plays an important role in the function of dendritic cells (DC), the specialized antigen-presenting cells of the immune system. Here we describe functional ryanodine receptor (RyR) Ca2+ release channels in murine, bone marrow-derived DC. RT-PCR analysis identified selective expression of the type 1 RyR, with higher levels detected in immature rather than mature DC. The RyR activators caffeine, FK506, ryanodine and 4-chloro-m-cresol mobilized Ca2+ in DC, and responses to 4-chloro-m-cresol were inhibited by dantrolene. Furthermore, activation of RyRs both inhibited subsequent inositol trisphosphate-mediated Ca2+ release and provoked store-operated Ca2+ entry, suggesting a functional interaction between these intracellular Ca2+ channels. Thus, the RyR1 channel may play an intrinsic role in Ca2+ signaling in DC.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020311416ZK.pdf | 126KB | download |