Identification of functional type 1 ryanodine receptors in mouse dendritic cells

FEBS Letters

Klyachko, Vitaly A.³  Ahern, Gerard P.¹  O'Connell, Peta J.² 

[1] Department of Pharmacology, Georgetown University, Washington DC, 20007, USA;The John P. Robarts Research Institute, London, ON, Canada N6A 5K8;Department of Physiology and Biophysics PhD Program, University of Wisconsin-Madison, Madison, WI 53706, USA

关键词: Dendritic cell;    Calcium;    Ryanodine receptor;    FK506;    Caffeine;   

DOI  :  10.1016/S0014-5793(01)03321-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ca²⁺ signaling plays an important role in the function of dendritic cells (DC), the specialized antigen-presenting cells of the immune system. Here we describe functional ryanodine receptor (RyR) Ca²⁺ release channels in murine, bone marrow-derived DC. RT-PCR analysis identified selective expression of the type 1 RyR, with higher levels detected in immature rather than mature DC. The RyR activators caffeine, FK506, ryanodine and 4-chloro-m-cresol mobilized Ca²⁺ in DC, and responses to 4-chloro-m-cresol were inhibited by dantrolene. Furthermore, activation of RyRs both inhibited subsequent inositol trisphosphate-mediated Ca²⁺ release and provoked store-operated Ca²⁺ entry, suggesting a functional interaction between these intracellular Ca²⁺ channels. Thus, the RyR1 channel may play an intrinsic role in Ca²⁺ signaling in DC.

【 授权许可】

Unknown