Cyclic ADP‐ribose‐induced Ca2+ release from rat brain microsomes

FEBS Letters

White, Alison M.¹  Watson, Stephen P.¹  Galione, Antony¹ 

[1] Department of Pharmacology, University of Oxford, Oxford, UK

关键词: Cyclic ADP-ribose;    Calcium;    Rat brain microsome;    Ryanodine receptor;   

DOI  :  10.1016/0014-5793(93)80524-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cyclic ADP-ribose (cADPR), an endogenous NAD⁺ metabolite in many mammalian and invertebrate tissues, is a potent mediator of calcium mobilization in sea urchin eggs. Our results show that cADPR also stimulates calcium release from rat brain microsomes, marked release occurring over the concentration range 10–250 nM. This is not inhibited by concentrations of heparin which completely abolish inositol 1,4,5-trisphosphate (IP₃)-induced Ca²⁺ release. Ryanodine (100μM) inhibits the cADPR response. Our results are consistent with cADPR being an endogenous messenger mediating Ca²⁺ release from ryanodine-sensitive pools in brain.

【 授权许可】

Unknown