Cyclic ADP‐ribose induced Ca2+ release in rabbit skeletal muscle sarcoplasmic reticulum

FEBS Letters

Morrissette, Jeffery¹  Ruoho, Arnold³  Coronado, Roberto¹  Heisermann, Gary²  Cleary, John³ 

[1] Departments of Physiology, University of Wisconsin School of Medicine, 1300 University Avenue, Madison, WI 53706, USA;Pacific Northwest Research Foundation, Seattle, WA, USA;Departments of Pharmacology, University of Wisconsin School of Medicine, 1300 University Avenue, Madison, WI 53706, USA

关键词: Cyclic ADP-ribose;    Ryanodine receptor;    Ca2+ release channel;   

DOI  :  10.1016/0014-5793(93)80886-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Ca²⁺-mobilizing metabolite cyclic ADP-ribose (cADPR) has been shown to release Ca²⁺ from ryanodine-sensitive stores in many cells. We show that this metabolite at a concentration of 17μM, but not its precursor β-NAD⁺ nor non-cyclic ADPR at the same concentration, is active in releasing Ca²⁺ from rabbit skeletal muscle sarcoplasmic reticulum. The release was not sensitive to Ruthenium red (1μM) nor to the ryanodine receptor-specific scorpion toxin Buthotus ₁-1 (10 μM). In planar bilayer single channel recordings, concentrations up to 50μM cADPR did not increase the open probability of Ruthenium red and toxin-sensitive Ca²⁺ release channels. Thus Ca²⁺ release induced by cADPR in skeletal muscle sarcoplasmic reticulum may not involve opening of ryanodine receptors.

【 授权许可】

Unknown