Sendai virus C protein impairs both phosphorylation and dephosphorylation processes of Stat1

FEBS Letters

Komatsu, Takayuki¹  Gotoh, Bin¹  Takeuchi, Kenji¹  Yokoo, Junko² 

[1] Department of Microbiology, Fukui Medical University School of Medicine, Shimoaizuki 23-3, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan;Radioisotope Research Institute, Fukui Medical University, Shimoaizuki 23-3, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan

关键词: Paramyxovirus;    Sendai virus;    Interferon;    Stat1;    Dephosphorylation;    Phosphorylation;    CBP;    cyclic AMP response element binding protein;    DMEM;    Dulbecco's minimum essential medium;    GAF;    γ-activated factor;    hPIV;    human parainfluenza virus;    IFN;    interferon;    ISG;    IFN-stimulated gene;    ISGF3;    IFN-stimulated gene factor 3;    ORF;    open reading frame;    PAGE;    polyacrylamide gel electrophoresis;    pi;    post-infection;    pS;    serine-phosphorylated;    pt;    post-treatment;    pY;    tyrosine-phosphorylated;    SDS;    sodium dodecyl sulfate;    SeV;    Sendai virus;    Stat;    signal transducer and activator of transcription;    SV;    simian virus;    WT;    wild-type;   

DOI  :  10.1016/S0014-5793(01)03301-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sendai virus expresses C protein that blocks interferon (IFN) signaling. We previously reported suppression of IFN-stimulated tyrosine phosphorylation of signal transducers and activators of transcription (Stats) in infected cells. However this conclusion has remained controversial. To settle it, we re-examined the effect of C protein expression on phosphorylation of Stat1 in detail. IFN-stimulated tyrosine phosphorylation of Stat1 was doubtlessly suppressed early in infection, but the suppression was incomplete, suggesting the importance of the unknown blocking mechanism that inactivates the tyrosine-phosphorylated (pY)-Stat1 generated as the signaling leak. Interestingly, the dephosphorylation process of pY-Stat1 was also impaired. These effects on both phosphorylation and dephosphorylation processes were attributable to the function of the C protein.

【 授权许可】

Unknown