Measles virus V protein blocks interferon (IFN)‐α/β but not IFN‐γ signaling by inhibiting STAT1 and STAT2 phosphorylation

FEBS Letters

Takeuchi, Kaoru³  Miyajima, Naoko²  Takeda, Makoto¹  Nagata, Kyosuke³  Kadota, Shin-ich³ 

[1] Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208-3500, USA;Department of Virology 3, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-murayama, Tokyo 208-0011, Japan;Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan

关键词: Paramyxovirus;    Measles virus;    Interferon;    V protein;    C protein;    STAT;    EMC;    encephalomyocarditis;    GAS;    γ-activated sequence;    IFN;    interferon;    ISGF3;    IFN-stimulated gene factor 3;    ISRE;    interferon-stimulated response element;    MV;    measles virus;    ORF;    open reading frame;    pS;    serine-phosphorylated;    pY;    tyrosine-phosphorylated;    SEAP;    secreted alkaline phosphatase;    STAT;    signal transducers and activators of transcription;    SV;    simian virus;   

DOI  :  10.1016/S0014-5793(03)00528-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Measles virus (MV), a member of the family Paramyxoviridae, encodes C and V non-structural proteins. To clarify the functions of MV C and V proteins, HeLa cell lines constitutively expressing C or V protein were established. We found that expression of V protein inhibited interferon (IFN)-α/β signaling but not IFN-γ signaling. C protein had no inhibitory effect on IFN signaling in our experimental condition. Degradation of selective signal transducers and activators of transcription (STAT) proteins was not observed in HeLa cells expressing V protein. In contrast, tyrosine phosphorylation of both STAT1 and STAT2 was inhibited in these cells after IFN-β stimulation.

【 授权许可】

Unknown