Peptides derived from the heptad repeat region near the C‐terminal of Sendai virus F protein bind the hemagglutinin‐neuraminidase ectodomain

FEBS Letters

Pasti, Claudia²  Dallocchio, Franco²  Tomasi, Maurizio¹  Manfrinato, Cristina²  Bellini, Tiziana² 

[1] Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy;Dipartimento di Biochimica e Biologia Molecolare, Università di Ferrara, Via Borsari 46, 44100 Ferrara, Italy

关键词: Sendai virus;    Paramyxovirus;    Neuraminidase;    Membrane fusion;    Viral membrane protein;    F;    fusion protein;    HN;    hemagglutinin-neuraminidase;    cHN;    soluble ectodomain of HN;    HR1;    heptad repeat region proximal to fusion peptide of F protein;    HR2;    heptad repeat region proximal to transmembrane domain of F protein;    NA;    neuraminidase activity;    HE;    hemolytic activity;   

DOI  :  10.1016/S0014-5793(03)00010-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previously, we showed that Sendai virus fusion protein (F) acts as an inhibitor of neuraminidase activity of hemagglutinin-neuraminidase (HN) protein. Here we report that synthetic peptides derived from the heptad repeat region proximal to the transmembrane domain (HR2) of Sendai virus F inhibit fusion and enhance the enzymatic activity of the HN. This occurs on the virus-bound HN and on its soluble globular head. The enhancing effect on virus-bound HN is reversible and depends on the presence of F. The data indicate that, by binding to the HN ectodomain, the HR2 peptides abolish the F inhibition of HN and disrupt the communication between the F and HN essential to promote virus–cell fusion.

【 授权许可】

Unknown