Selective extraction of haemagglutinin and matrix protein from Sendai virions by employing trifluoperazine as a detergent

FEBS Letters

Montecucco, Cesare²  Bruschi, Maria Letizia¹  Tomasi, Maurizio¹  Baiocchi, Marta¹  Pescarmona, Marina¹  Squatriti, Teodoro¹ 

[1]Laboratorio di Biologia Cellulare, Istituto Superiore di Sanita, V. le Regina Elena 299, 00161 Roma Italy

[2]Istituto di Patologia Generale, Università degli studi di Padova, Via Loredan 56, 3500 Padova, Italy

关键词: Sendai virus;    Fusion;    Membrane protein;    Neuraminidase;    Phenothiazine;    Surfactant;    HN;    haemagglutinin neuraminidase glycoprotein;    F;    fusogenic glycoprotein;    M;    matrix protein;    SV;    Sendai virus;    TFP;    trifluoperazine;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(88)80250-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of trifluoperazine, a local anaesthetic, at concentrations higher than the cmc with Sendai virus particles produces the selective solubilization of the haemagglutinin neuraminidase (HN) and matrix (M) proteins. This phenomenon involves aggregation of the Sendai virions and therefore the separation of HN and M from the rest of the particle can be performed by bench centrifugation. The supernatant contains the HN and M proteins and HN, once inserted into liposomes, elicits its own biological activities. Therefore, the method seems suitable for purifying large amounts of HN.

【 授权许可】

Unknown