Interaction of Sendai virions with resealed human erythrocyte ghosts Lateral mobility of the viral glycoproteins in the cell membrane following fusion

FEBS Letters

Gutman, Orit¹  Henis, Yoav I.¹ 

[1] Department of Biochemistry, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel

关键词: Fusion;    Sendai virus;    Photobleaching;    Diffusion;    Erythrocyte;   

DOI  :  10.1016/0014-5793(88)80016-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two independent methods demonstrated that resealed human erythrocyte ghosts undergo Sendai virus-mediated cell-cell fusion to a much lower degree (about 4%) than intact erythrocytes, in spite of similar levels of viral envelope-cell fusion in the two preparations. Fluorescence photobleaching recovery (FPR) showed similar lateral mobilities of the viral glycoproteins following fusion with either ghosts or whole erythrocytes. It is suggested that although viral glycoprotein mobilization in the cell membrane is essential for cell-cell fusion, the target cell properties are also important; in the absence of the required cellular parameters, the mobilization may not be a sufficient condition.

【 授权许可】

Unknown