Cytotoxicity acquired by ribosome‐inactivating proteins carried by reconstituted Sendai virus envelopes

FEBS Letters

Tomasi, Maurizio¹  Sargiacomo, Massimo¹  Barbieri, Luigi²  Stirpe, Fiorenzo² 

[1] Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy;Istituto di Patologia Generale dell'Università di Bologna, 40126 Bologna, Italy

关键词: Ribosome inactivating protein;    Sendai virus;    Toxin;    Microinjection;    Hybrid;    Fusion;    CHO;    Chinese hamster ovary;    DTA;    diphtheria toxin A-chain;    DTT;    dithiothreitol;    ID 50;    concentration causing 50% inhibition;    MCI;    Momordica charantia inhibitor;    PAP;    pokeweed antiviral protein (prepared from seeds;    PAP-S [15]);    PMSF;    p-methylsulphonylfluoride;    RIP;    ribosome-inactivating protein;    RSVE;    reconstituted Sendai virus envelope;   

DOI  :  10.1016/0014-5793(83)81135-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Association of the ribosome-inactivating proteins (RIPs): pokeweed antiviral protein (PAP), gelonin, Momordica charantia inhibitor (MCI), with reconstituted Sendai virus envelopes (RSVE) was obtained without detectable loss of activities either of RIPs or of viral envelope glycoproteins. RIPs are inactive towards intact cells, but, once encapsulated in RSVE, they become cytotoxic. The concentration of RSVE-associated PAP, which causes 50% inhibition of protein synthesis by Friend erythroleukemic cells, is 0.5 ng/ml. Substances capable to inhibit the viral activities block the acquired cytotoxicity of RIPs associated to RSVE.

【 授权许可】

Unknown