Knockout of the Sendai virus C gene eliminates the viral ability to prevent the interferon‐α/β‐mediated responses

FEBS Letters

Kurotani, Atsushi¹  Komatsu, Takayuki³  Gotoh, Bin³  Kimura, Yoshinobu³  Nagai, Yoshiyuki¹  Kato, Atsushi⁴  Takeuchi, Kenji³  Yokoo, Junko² 

[1] Department of Viral Infection, Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo 108-8639, Japan;Radioisotope Research Institute, Fukui Medical University, Shimoaizuki 23-3, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan;Department of Microbiology, Fukui Medical University, Shimoaizuki 23-3, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan;Department of Viral Diseases and Vaccine Control, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan

关键词: Sendai virus;    C protein;    Interferon;    Interferon-stimulated gene;    Stat1;    p48;   

DOI  :  10.1016/S0014-5793(99)01241-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sendai virus (SeV) renders cells unresponsive to interferon (IFN)-α. To identify viral factors involved in this process, we examined whether recombinant SeVs, which could not express V protein, subsets of C proteins (C, C′, Y1 and Y2) or any of four C proteins, retained the capability of impeding IFN-α-mediated responses. Among these viruses, only the 4C knockout virus completely lost the ability to suppress the induction of IFN-α-stimulated gene products and the subsequent establishment of an anti-viral state. These findings reveal crucial roles of the SeV C proteins in blocking IFN-α-mediated responses.

【 授权许可】

Unknown