Identification of two novel interferon-stimulated genes from cultured CAB cells induced byUV-inactivated grass carp hemorrhage virus

Diseases of Aquatic Organisms

Yi Bing Zhang¹  Jian Fang Gui¹ 

关键词: Interferon;    Interferon-stimulated gene;    Antiviral-relevant gene;    JAK-STAT signal pathway;    Crucian carp embryonic blastulae cells;    CAB;    Grass carp hemorrhage virus;   

DOI  :  10.3354/dao060001

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Interferon (IFN) exerts its antiviral effect by inducing the expression of a number of IFN-stimulated genes (ISGs) to establish a host antiviral state. Earlier studies identified some important fish IFN system genes from IFN-induced CAB cells(crucian carp Carassius auratus L. embryonic blastulae cells) after treatment with UV-inactivated GCHV (grass carp hemorrhage virus). Herein, the cloning of 2 novel IFN-stimulated genes, termed Gig1 and Gig2, is described for the same cell system.The complete cDNA sequences of Gig1 and Gig2 contain 1244 bp encoding for a 194-amino-acid protein and 693 bp for a 158-amino-acid protein, respectively. A search of public databases revealed that these are 2 novel IFN-stimulated genes, since neithersignificant homologous genes nor conserved motifs were identified. Active GCHV, UV-inactivated GCHV and CAB IFN-containing supernatant (ICS) induced transcription of these genes and distinct kinetics were observed. An analysis of differences inexpression between the 2 genes and the IFN signal factors CaSTAT1 and CaIRF7 indicated that GCHV infection activated different signal pathways for their up-regulation. Upon virus infection, the transcription of Gig1 but not of Gig2 is strongly suppressedby cycloheximide (CHX). In contrast, following treatment with CAB IFN-containing supernatant, CHX does not inhibit either gene transcription. The results suggest that GCHV infection can induce expression of both Gig1 and Gig2 via newly synthesizedCAB IFN, most probably through the JAK-STAT signal pathway, and can also directly activate Gig2 transcription without ongoing protein synthesis.

【 授权许可】

Unknown