Change in the responsiveness of interferon-stimulated genes during early pregnancy in cows with Borna virus-1 infection

BMC Veterinary Research

Research Article

Katsuro Hagiwara¹  Taku Okamura¹  Tadashi Takino²  Tatsuya Ando³ 

[1] School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan;School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan;Scientific Feed Laboratory Co., Ltd., 3-5 Miyahara, Takasaki, Gunma, Japan;Veterinary Clinical Center, NOSAI Hokkaido, Ishikari district, Japan;

关键词: Interferon-stimulated gene;    IFN-τ;    pregnant;    Borna disease virus;   

DOI  :  10.1186/s12917-016-0883-5

 received in 2016-06-13, accepted in 2016-11-09,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBorna disease virus is a neurotropic pathogen and infects the central nervous system. This virus infected a variety of animal species including cows. The most of cows infected with Borna disease virus 1 (BoDV-1) exhibit subclinical infection without any neurological symptoms throughout their lifetime. We previously reported on the low conception rates in-seropositive cows. Interferon-τ (IFN-τ) plays an important role in stable fertilization, and is produced from the fetal side following embryo growth at 15–40 days of pregnancy. IFN-τ induces the expression of interferon-stimulated gene (ISG) 15 and Mx2 in peripheral blood mononuclear cells (PBMCs). To understand the embryo growth and maternal reaction during early pregnancy in cows with BoDV-1 infection, we aimed to assess the gene expression of ISG15 and Mx2 from PBMCs in BoDV-1-seropositive cows.ResultsNone of the cows showed any clinical and neurological symptoms. Among the cows that conceived, the expressions of the ISG15 and Mx2 genes were greater in the BoDV-1-seropositive cows than in the BoDV-1-seronegative cows; the difference was significant between the cows that conceived and those that did not (P < 0.05).ConclusionsThe expression of ISG15 and Mx2 genes during early pregnancy significantly increased in the BoDV-1-seropositive cows and may be important for the maintenance of stable pregnancy in BoDV-1-infected cows. In contrast, the gene expression levels of ISG15 and Mx2 did not significantly increase during early pregnancy in BoDV-1-seronegative cows. Thus, BoDV-1 infection may lead to instability in the maintenance of early pregnancy by interfering with INF-τ production.

【 授权许可】

CC BY   
© The Author(s). 2016