A strategy for the generation of non‐aggregating mutants of Anthozoa fluorescent proteins

FEBS Letters

Fradkov, Arkady F.¹  Bulina, Maria E.¹  Staroverov, Dmitry B.¹  Savitsky, Alexander P.²  Yanushevich, Yurii G.¹  Gurskaya, Nadya G.¹  Lukyanov, Sergey A.¹  Lukyanov, Konstantin A.¹ 

[1]Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, 117997 Moscow, Russia

[2]Institute of Biochemistry RAS, Leninsky pr. 33, 117071 Moscow, Russia

关键词: Green fluorescent protein;    DsRed;    Mutagenesis;   

DOI  :  10.1016/S0014-5793(01)03263-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recently, we cloned several fluorescent proteins of different colors homologous to Aequorea victoria green fluorescent protein, which have great biotechnological potential as in vivo markers of gene expression. However, later investigations revealed severe drawbacks in the use of novel fluorescent proteins (FPs), in particular, the formation of tetramers (tetramerization) and high molecular weight aggregates (aggregation). In this report, we employ a mutagenic approach to resolve the problem of aggregation. The elimination of basic residues located near the N-termini of FPs results in the generation of non-aggregating versions of several FPs, specifically, drFP583 (DsRed), DsRed-Timer, ds/drFP616, zFP506, zFP538, amFP486, and asFP595.

【 授权许可】

Unknown