Interleukin‐1β stimulates cyclic GMP efflux in brain astrocytes

FEBS Letters

Garcı́a, Agustina¹  Pedraza, Carlos E¹  Baltrons, Marı́a Antonia¹ 

[1] Instituto de Biotecnologı́a y Biomedicina ‘V. Villar Palası́’ and Departamento de Bioquı́mica y Biologı́a Molecular, Universidad Autónoma de Barcelona, Bellaterra (Barcelona), Spain

关键词: cGMP efflux;    Nitric oxide;    Interleukin-1β;    Astroglia;    DMEM;    Dulbecco's modified Eagle's medium;    FCS;    fetal calf serum;    IL-1β;    interleukin-1β;    IL-1Ra;    IL-1 receptor antagonist;    INF-γ;    interferon-γ;    LDH;    lactate dehydrogenase;    L-NMMA N G-monomethyl-L-arginine;    LPS;    lipopolysaccharide;    NOS;    NO synthase;    sGC;    soluble guanylyl cyclase;    TNF-α;    tumor necrosis factor-α;    ODQ;    1H-[1;    2;    4]oxodiazolo-[4;    3-a]quinoxalin-1-one;   

DOI  :  10.1016/S0014-5793(01)03003-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In rat brain astroglia-enriched cultures long-term treatment with interleukin-1β induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1β effect is mediated by type I receptor and potentiated by interferon-γ. In cells treated with bacterial endotoxin a larger NO-dependent cGMP accumulation occurs only intracellularly, however a significant cGMP egression is observed when cells are co-treated with interleukin-1β. The non-selective anion transport inhibitors probenecid and verapamil block cGMP efflux, indicating that interleukin-1β stimulates a cGMP transporter.

【 授权许可】

Unknown