Interleukin‐1 ‐induced expression of nitric oxide synthase in insulin‐producing cells is preceded by c‐fos induction and depends on gene transcription and protein synthesis

FEBS Letters

Björklund, Andreas¹  Welsh, Nils¹  Eizirik, Décio L.¹ 

[1] Department of Medical Cell Biology, Uppsala University, Uppsala S-751 23, Sweden

关键词: Interleukin-1β;    Nitric oxide;    Nitric oxide synthase;    HIT cell;    Insulin-producing cell;    Insulin-dependent diabetes mellitus;   

DOI  :  10.1016/0014-5793(93)81492-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cytokine interleukin 1β (IL-1β) induces the expression of an isoform of nitric oxide synthase (NOS) in insulin-producing cells which is similar to that expressed in activated macrophages. In the present study we show that IL-1β-induced expression of NOS mRNA in these cells is preceded by expression of c-fos mRNA. Moreover, the stimulatory effects of recombinant IL-1β on NOS mRNA expression are prevented by co-incubation with an inhibitor of gene transcription (actinomycin D) or an inhibitor of protein synthesis cycloheximide). These data suggest that IL-1β-induced NOS mRNA expression may be mediated by transcription of immediate early response genes, and that c-fos may be one of these genes.

【 授权许可】

Unknown