Insulin‐like growth factor I reverses interleukin‐1β inhibition of insulin secretion, induction of nitric oxide synthase and cytokine‐mediated apoptosis in rat islets of Langerhans

FEBS Letters

Green, Irene C.¹  Belin, Véronique¹  John, Nerys¹  Mabley, Jon G.¹ 

[1] Department of Biochemistry, University of Sussex, Falmer, Brighton BN1 9QG, UK

关键词: Insulin-like growth factor I;    Interleukin-1β;    Nitric oxide synthase;    Islet of Langerhans;    Apoptosis;    Insulin secretion;    IGF-I;    insulin-like growth factor I;    IL-1β;    interleukin-1β;    TNF-α;    tumour necrosis factor-α;    IFN-γ;    interferon-γ;    TGFβ1;    transforming growth factor β1;    iNOS;    inducible nitric oxide synthase;   

DOI  :  10.1016/S0014-5793(97)01291-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously observed that treatment of rat islets of Langerhans with interleukin-1β for 12 h results in nitric oxide-dependent inhibition of insulin secretion, while 48 h treatment increased rates of islet cell death by apoptosis. Here, we demonstrate that interleukin-1β-mediated nitric oxide formation and inhibition of insulin secretion are significantly reduced by 24 h pretreatment of rat islets of Langerhans with insulin-like growth factor I (IGF-I). IGF-I decreased cytokine induction of nitric oxide synthase in islets. Use of an arginine analogue in culture or IGF-I pretreatment of islets were also effective in protecting islets against cytokine-mediated apoptotic cell death. We conclude that IGF-I antagonises inhibitory and cytotoxic effects of cytokines in rat islets.

【 授权许可】

Unknown