Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing

Molecular Metabolism

Amir Giladi¹  Nancy S. Yacovzada¹  Ido Amit²  Lydia Farack³  Diego Jaitin³  Adi Egozi³  Danny Ben-Zvi⁴  Eran Hornstein⁴  Shalev Itzkovitz⁴  Eran Yanowski⁵  Eyal David⁵ 

[1] Department of Molecular Neuroscience, Weizmann Institute of Science, Rehovot 7610001, Israel;Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, 9112102, Israel;Department of Immunology, Weizmann Institute of Science, Rehovot 7610001, Israel;Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 7610001, Israel;Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel;

关键词: Islet of Langerhans;    Endocrine pancreas;    Single-cell RNA-sequencing;    Single-cell transcriptome sequencing;    scRNA-seq;    beta-delta cell pair;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: Until recently, communication between neighboring cells in islets of Langerhans was overlooked by genomic technologies, which require rigorous tissue dissociation into single cells. Methods: We utilize sorting of physically interacting cells (PICs) with single-cell RNA-sequencing to systematically map cellular interactions in the endocrine pancreas after pancreatectomy. Results: The pancreas cellular landscape features pancreatectomy associated heterogeneity of beta-cells, including an interaction-specific program between paired beta and delta-cells. Conclusions: Our analysis suggests that the particular cluster of beta-cells that pairs with delta-cells benefits from stress protection, implying that the interaction between beta- and delta-cells might safeguard against pancreatectomy associated challenges. The work encourages testing the potential relevance of physically-interacting beta-delta-cells also in diabetes mellitus.

【 授权许可】

Unknown