Interleukin‐1β induces the expression of an isoform of nitric oxide synthase in insulin‐producing cells, which is similar to that observed in activated macrophages

FEBS Letters

Björklund, Andres¹  Cagliero, Enrico²  Welsh, Nils¹  Eizirik, Décio L.¹ 

[1] Department of Medical Cell Biology, Uppsala University, Uppsala S 751 23, Sweden;Schepens Eye Research Institute and Department of Ophatalmology, Harvard Medical School, Boston, MA, USA

关键词: Interleukin-1β;    Nitric oxide: Nitric oxide synthase;    Pancreatic islet;    HIT cell;    Insulin-dependent diabetes mellitus;   

DOI  :  10.1016/0014-5793(92)81285-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The suppressive and cytotoxic effects of interleukin-1β(IL-β) on rodent insulin-producing cells observed in vitro are probably mediated through formation or nitric oxide (NO). In this study we demonstrate that IL-1-induced NO formation in isolated rat islets and insulin-producing HIT cells is more sensitive to inhibition by N ^G-monomethyl-l-arginine than to inhibition by N ^G-nitro-l-arginine, thus suggesting that IL-1-exposed insulin producing cells express an isoform of nitric oxide synthase similar to that present in activated macrophages, Furthermore, IL-1β markedly increased the mRNA levels or the inducible microphage form of nitric oxide synthase in HIT cells.

【 授权许可】

Unknown