Endogenous nitric oxide induced by interleukin‐1β in rat islets of Langerhans and HIT‐T15 cells causes significant DNA damage as measured by the ‘comet’ assay

FEBS Letters

Delaney, C.A.²  Lowe, J.E.¹  Green, I.C.²  Green, M.H.L.¹ 

[1] MRC Cell Mutation Unit, University of Sussex, Brighton, BN1 9RR, UK;Department of Biochemistry, School of Biological Sciences, University of Sussex, Brighton, BN1 9QG, UK

关键词: Interleukin-1β;    Nitric oxide;    DNA damage;    Islets of Langerhans;    HIT-T15 cell;    Comet assay (single cell gel electrophoresis);    Peroxynitrite;   

DOI  :  10.1016/0014-5793(93)80673-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have used the comet assay (single cell gel electrophoresis) to measure nitric oxide-induced DNA damage in rat islets of Langerhans and insulin-containing HIT-T15 cells. Damage was induced following treatment with the nitric oxide donor SIN-1, which also releases Superoxide, but was not reduced by exogenous Superoxide dismutase, suggesting that nitric oxide itself, rather than Superoxide or peroxynitrite may be the active species. The DNA damaging effect of nitric oxide was easily detectable at the earliest time point tested (15 min). Damage also resulted following induction of nitric oxide synthase by the cytokine interleukin-1β in both islets and HIT-T15 cells and was prevented by replacing the substrate, arginine, with nitromonomethyl arginine. Thus intracellular levels of nitric oxide generated by interleukin-1β-induced nitric oxide synthase were sufficient to cause DNA damage in islet cells and HIT-T15 cells.

【 授权许可】

Unknown