Semliki Forest virus vectors: efficient vehicles for in vitro and in vivo gene delivery

FEBS Letters

Ehrengruber, Markus U.²  Schneider, Edith M.²  Hermann, Danielle¹  Schweitzer, Christophe¹  Rotmann, Daniel¹  Lundstrom, Kenneth¹ 

[1] F. Hoffmann-La Roche, CNS Department, CH-4070 Basel, Switzerland;Brain Research Institute, University of Zurich, CH-8057 Zurich, Switzerland

关键词: Semliki Forest virus vector;    Recombinant receptor;    Protein purification;    Neuronal expression;    In vivo gene delivery;    Hippocampus;    Connexin 36;    BHK;    baby hamster kidney;    GFP;    green fluorescent protein;    GPCR;    G protein-coupled receptor;    hNK1R;    human neurokinin-1 receptor;    SFV;    Semliki Forest virus;   

DOI  :  10.1016/S0014-5793(01)02707-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rapidly generated high-titer Semliki Forest virus (SFV) vectors can infect numerous mammalian cell lines and primary cell cultures, and result in high levels of transgene expression. SFV-based expression of transmembrane receptors has been characterized by specific ligand-binding activity and functional responses. Adaptation of the SFV technology for mammalian suspension cultures has allowed the production of hundreds of milligrams of recombinant receptor for purification and structural studies. The same SFV stock solutions used for the infection of mammalian cells in culture have also been successfully applied for efficient transgene expression in organotypic hippocampal slices, as well as in vivo in rodent brain.

【 授权许可】

Unknown