Functional characterization of the promoter region of the mouse protein kinase C γ gene

FEBS Letters

Mukai, Hideyuki³  Shimakawa, Masaki³  Konishi, Hiroaki¹  Kikkawa, Ushio¹  Koizumi, Tamio²  Takanaga, Hiromi³  Ono, Yoshitaka³ 

[1] Biosignal Research Center, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657, Japan;Hyogo Institute of Clinical Research, 13-77 Kitaoji-cho, Akashi 673, Japan;Department of Biology, Faculty of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657, Japan

关键词: PKC γ;    Neuronal expression;    Promoter;    PC12 cell;    bp;    base pair(s);    kb;    kilobase(s);    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(95)00665-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Promoter activity of protein kinase C (PKC) γ gene was analysed by chloramphenicol acetyltransferase (CAT) assay using extracts from the cells transfected with various fusion constructs containing the 5′-flanking region of the mouse PKC γ gene and CAT gene. Transient expression experiments in PC12 cells revealed that the upstream region of 87 bp from the transcriptional initiation site was sufficient for promoter activity. The region containing nucleotides 87 upstream from the transcriptional initiation site was shown to silence CAT activity in Balb/c3T3 cells, in which mRNA of PKC γ was not detected, suggesting that this region might contain a transcriptional regulatory element for the cell type-specific expression of the PKC γ gene.

【 授权许可】

Unknown