Apolipoprotein E isoform‐specific disruption of phosphoinositide hydrolysis: protection by estrogen and glutathione

FEBS Letters

Cowburn, Richard F¹  Cedazo-Mı́nguez, Angel¹ 

[1] Karolinska Institutet, NEUROTEC, Division of Experimental Geriatrics, NOVUM, KFC, 141 86 Huddinge, Sweden

关键词: Alzheimer's disease;    Apolipoprotein E;    β-Amyloid;    Phosphoinositide;    Estrogen;    Glutathione;    Aβ;    β-amyloid;    AD;    Alzheimer's disease;    AO;    antioxidants;    apoE;    apolipoprotein E;    GSH;    glutathione (reduced form);    HNE;    4-hydroxynonenal;    KHB;    Krebs–Henseleit bicarbonate buffer;    MEM;    minimum essential medium;    PG;    n-propyl gallate (3;    4;    5-trihydroxybenzoic acid n-propyl ester);    PI;    phosphoinositide;    PI3K;    phosphatidylinositol 3-kinase;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    PIP3;    phosphatidylinositol 3;    4;    5-trisphosphate;    PLC;    phospholipase C;   

DOI  :  10.1016/S0014-5793(01)02761-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mechanism(s) by which the E4 isoform of apolipoprotein E (apoE4) influences Alzheimer's disease (AD) are not fully known. We report that apoE4, but not apoE3, disrupts carbachol-stimulated phosphoinositide (PI) hydrolysis in SH-SY5Y neuroblastoma cells. Carbachol responses were also disrupted by β-amyloid (Aβ) (1–42) and apoE4/Aβ(1–42) complexes, but not by apoE3/Aβ(1–42). Glutathione and estrogen protected against apoE4 and Aβ(1–42) effects, as well as those of H₂O₂. Estrogen protection was partially blocked by wortmannin, suggesting the involvement of phosphatidylinositol 3-kinase. An apoE4-induced disruption of acetylcholine muscarinic receptor-mediated signalling may explain the lower effectiveness of cholinergic replacement treatments in apoE4 AD patients. Also, the beneficial effect of estrogen in AD may be partially due to its ability to protect against apoE4- and Aβ(1–42)-mediated disruption of PI hydrolysis.

【 授权许可】

Unknown