Alzheimer's β‐amyloid peptide specifically interacts with and is degraded by insulin degrading enzyme

FEBS Letters

Goto, Sataro¹  Kurochkin, Igor V.¹ 

[1] Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Miyama, Funabashi, Chiba 274, Japan

关键词: Alzheimer's disease;    β-Amyloid;    Insulin degrading enzyme;    Oxidative modification;    Protein degradation;    Brain;    Rat;   

DOI  :  10.1016/0014-5793(94)00387-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cerebral deposition of β-amyloid peptide (βA) is a hallmark of Alzheimer's disease. Concentration of βA could play a critical role in the rate of amyloid deposition. It is therefore of considerable importance to identify proteases involved in processing of βA. ¹²⁵I-labeled synthetic βA specifically cross-linked to a single protein with M _r = 110,000 in cytosol fractions from rat brain and liver. This protein was identified as insulin degrading enzyme (IDE) since the labeling of the 110 kDa protein was completely blocked by an excess of insulin, and anti-IDE monoclonal antibodies precipitated the labeled protein. Purified rat IDE effectively degraded βA.

【 授权许可】

Unknown