Functional disruption of IEX‐1 expression by concatemeric hammerhead ribozymes alters growth properties of 293 cells

FEBS Letters

Krupp, Guido¹  Ungefroren, Hendrik⁴  Arlt, Alexander²  Grobe, Olaf²  Schäfer, Heiner²  Fölsch, Ulrich R.²  Schmidt, Wolfgang E.³ 

[1] Department of Haematopathology, University of Kiel, Kiel, Germany;Laboratory of Molecular Gastroenterology, First Department of Internal Medicine, University of Kiel, Schittenhelmstr. 12, D-24105 Kiel, Germany;Medical Department 1, St. Josef-Hospital, University of Bochum, Bochum, Germany;Molecular Oncology Group, Department of General Surgery, University of Kiel, Kiel, Germany

关键词: Cell cycle;    Apoptosis;    Antisense;    Hammerhead ribozyme;    IEX-1;    immediate early gene on X-radiation 1;    MTS;    3-(4;    5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt;    PI;    propidium iodide;    FCS;    foetal calf serum;    DMEM;    Dulbecco's minimal essential medium;   

DOI  :  10.1016/S0014-5793(01)02344-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably transfected with a concatemeric ribozyme construct. In addition, these 293 cells were much less sensitive to apoptosis induced by an activating Fas/CD95 antibody or by the anti-cancer drugs etoposide and doxorubicin. By modulating the cell cycle, IEX-1 might be part of a growth signal if favourable growth conditions prevail, whereas under unfavourable conditions, i.e. death receptor activation, IEX-1 facilitates apoptosis.

【 授权许可】

Unknown