Up‐regulation of uncoupling proteins by β‐adrenergic stimulation in L6 myotubes

FEBS Letters

Nagase, Itsuro¹  Saito, Masayuki¹  Yoshida, Toshihide² 

[1] Department of Biomedical Sciences, Laboratory of Biochemistry, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan;First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan

关键词: Uncoupling protein;    β-Adrenergic receptor;    L6 myotube;    Skeletal muscle;    BAT;    brown adipose tissue;    AR;    adrenergic receptor;    UCP;    uncoupling protein;    PPAR;    peroxisome proliferator-activated receptor;    RXR;    retinoid X receptor;    GAPDH;    glyceraldehyde 3-phosphate dehydrogenase;    PGC-1;    PPAR gamma coactivator-1;    CRE;    cAMP response element;    PKA;    cAMP-dependent protein kinase;   

DOI  :  10.1016/S0014-5793(01)02341-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Catecholamine-induced and β-adrenergic receptor (β-AR)-mediated thermogenesis in skeletal muscle is a significant component of whole-body energy expenditure. Skeletal muscle expresses uncoupling protein (UCP) 2 and UCP3, which can dissipate the transmitochondrial electrochemical gradient and thereby may be involved in regulation of energy metabolism. We investigated the effects of β-AR stimulation on UCP2 and UCP3 expression in L6 myotubes. Stimulation of the cells with epinephrine increased the UCP3 mRNA level transiently at 6 h, and also the UCP2 mRNA level at 6–24 h. The stimulatory effects of epinephrine were also observed in the presence of carbacyclin and 9-cis retinoic acid, and mimicked by isoproterenol and salbutamol (β2-AR agonists), but abolished by propranolol and ICI-118,551 (β2-AR antagonists). Pharmacological and mRNA analyses revealed the existence of β2-AR, but not β1- and β3-ARs, in L6 myotubes. These results suggested that catecholamines up-regulate UCP2 and UCP3 expression through direct action on the β2-AR in skeletal muscle.

【 授权许可】

Unknown