FEBS Letters | |
Up‐regulation of uncoupling proteins by β‐adrenergic stimulation in L6 myotubes | |
Nagase, Itsuro1  Saito, Masayuki1  Yoshida, Toshihide2  | |
[1] Department of Biomedical Sciences, Laboratory of Biochemistry, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan;First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan | |
关键词: Uncoupling protein; β-Adrenergic receptor; L6 myotube; Skeletal muscle; BAT; brown adipose tissue; AR; adrenergic receptor; UCP; uncoupling protein; PPAR; peroxisome proliferator-activated receptor; RXR; retinoid X receptor; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; PGC-1; PPAR gamma coactivator-1; CRE; cAMP response element; PKA; cAMP-dependent protein kinase; | |
DOI : 10.1016/S0014-5793(01)02341-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
Catecholamine-induced and β-adrenergic receptor (β-AR)-mediated thermogenesis in skeletal muscle is a significant component of whole-body energy expenditure. Skeletal muscle expresses uncoupling protein (UCP) 2 and UCP3, which can dissipate the transmitochondrial electrochemical gradient and thereby may be involved in regulation of energy metabolism. We investigated the effects of β-AR stimulation on UCP2 and UCP3 expression in L6 myotubes. Stimulation of the cells with epinephrine increased the UCP3 mRNA level transiently at 6 h, and also the UCP2 mRNA level at 6–24 h. The stimulatory effects of epinephrine were also observed in the presence of carbacyclin and 9-cis retinoic acid, and mimicked by isoproterenol and salbutamol (β2-AR agonists), but abolished by propranolol and ICI-118,551 (β2-AR antagonists). Pharmacological and mRNA analyses revealed the existence of β2-AR, but not β1- and β3-ARs, in L6 myotubes. These results suggested that catecholamines up-regulate UCP2 and UCP3 expression through direct action on the β2-AR in skeletal muscle.
【 授权许可】
Unknown
【 预 览 】
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