UCP2 and UCP3 rise in starved rat skeletal muscle but mitochondrial proton conductance is unchanged

FEBS Letters

Buckingham, Julie A.³  Din, Nanni⁴  Dulloo, Abdul G.¹  Samec, Sonia²  Brand, Martin D.³  Seydoux, Josiane²  Cadenas, Susana³ 

[1] Institute of Physiology, University of Fribourg, Fribourg, Switzerland;Department of Physiology, Faculty of Medicine, University of Geneva, 1211 Geneva 4, Switzerland;Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK;Department of Molecular Genetics, Novo Nordisk A/S, DK-2880 Bagsvaerd, Denmark

关键词: Uncoupling protein;    UCP2;    UCP3;    Fasting;    Proton leak;    Skeletal muscle;   

DOI  :  10.1016/S0014-5793(99)01540-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relationship between UCP2 and UCP3 expression and mitochondrial proton conductance of rat skeletal muscle was examined. Rats were starved for 24 h and the levels of UCP2 and UCP3 mRNA and UCP3 protein were determined by Northern and Western blots. Proton conductance was measured by titrating mitochondrial respiration rate and membrane potential with malonate. Starvation increased UCP2 and UCP3 mRNA levels more than 5-fold and 4-fold, respectively, and UCP3 protein levels by 2-fold. However, proton conductance remained unchanged. These results suggest either that Northern and Western blots do not reflect the levels of active protein or that these UCPs do not catalyse the basal proton conductance in skeletal muscle mitochondria.

【 授权许可】

Unknown